Journal: International Journal of Molecular Sciences
Article Title: PEG Linker Length Strongly Affects Tumor Cell Killing by PEGylated Carbonic Anhydrase Inhibitors in Hypoxic Carcinomas Expressing Carbonic Anhydrase IX
doi: 10.3390/ijms22031120
Figure Lengend Snippet: ( a ) Expression of CA IX target in 2D (normoxic/hypoxic) and 3D cancer cell cultures and the effect of PEGylated CAIs DTP2K 23 and DTP1K 28–DTP20K 31 and acetazolamide AAZ 1 at different concentrations on the viability of CA IX expressing cell lines (HT-29, MDA-MB231, and SKOV-3) and CA IX-negative cell line (NCI-H23) under normoxic ( b ) and hypoxic ( c ) conditions (all 2D cell cultures): the p values were determined by one-way ANOVA, comparing the value with acetazolamide 1 (* p < 0.05, ** p < 0.01, and *** p < 0.001). Only the statistically significant differences were indicated.
Article Snippet: Human colon adenocarcinoma cell line (HT-29), human ovary cancer cell line (SKOV-3-Luc), human breast cancer cell line (MDA-MB-231-Luc) and lung cancer cell line NCI-H23 were purchased from ATCC (Manassas, VA, USA).
Techniques: Expressing